Purified HrpA ofPseudomonas syringaepv.tomatoDC3000 reassembles into pili
نویسندگان
چکیده
منابع مشابه
Hemagglutination by purified type I Escherichia coli pili
Many enterobacteria can cause agglutination of erythrocytes, but previous investigations have not proven which components of the bacteria are responsible. We used a strain of Escherichia coli K12 which causes mannose-sensitive hemagglutination (HA) of guinea pig cells. Common pili were purified from these bacteria by shearing them from the bacteria followed by selective precipitation in acid an...
متن کاملComposition and molecular weight of pili purified from Pseudomonas aeruginosa K.
Pseudomonas aeruginosa strain K (PAK) bears polar pili that promote infection by at least six bacteriophages. Moreover, a recently isolated mutant of strain K (PAK/2PfS) is many times more piliated than the wild-type strain and facilitates the preparation of large amounts of pure pili for biochemical studies. The present investigation was carried out to establish the structural relatedness of P...
متن کاملPurified prion proteins and scrapie infectivity copartition into liposomes.
Considerable evidence indicates that the scrapie prion protein (PrP 27-30) is required for infectivity. Aggregates of PrP 27-30 form insoluble amyloid rods that resist dissociation by nondenaturing detergents. Mixtures of the detergent cholate and phospholipids were found to solubilize purified PrP 27-30 in the form of detergent-lipid-protein complexes. Removal of the cholate by dialysis result...
متن کاملFunctional Reconstitution into Liposomes of Purified Human RhCG Ammonia Channel
BACKGROUND Rh glycoproteins (RhAG, RhBG, RhCG) are members of the Amt/Mep/Rh family which facilitate movement of ammonium across plasma membranes. Changes in ammonium transport activity following expression of Rh glycoproteins have been described in different heterologous systems such as yeasts, oocytes and eukaryotic cell lines. However, in these complex systems, a potential contribution of en...
متن کاملSaturable acetylcholine transport into purified cholinergic synaptic vesicles.
The uptake of [3H[acetylcholine ([3H]AcCho) into cholinergic synaptic vesicle ghosts purified from Torpedo electric organ was studied at concentration of [3H]AcCho ranging from 0.1 to 10 mM. The accumulated [3H]AcCho can be released either by hypoosmotic buffer or by low levels of the detergent Triton X-100. Kinetic analysis of the initial rate of [3H]AcCho uptake reveals temperature-dependent ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1997
ISSN: 0014-5793
DOI: 10.1016/s0014-5793(97)01276-3